Formulation and Evaluation of Coumarin Loaded Transferosomal Gel
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Abstract
Coumarin is a naturally occurring phytochemical that exhibits various pharmacological activities, including anti-inflammatory, antioxidant, antimicrobial and wound-healing properties. However, its topical therapeutic effectiveness is limited due to poor aqueous solubility and inadequate skin permeation. Transferosomes are highly deformable lipid vesicles capable of penetrating the stratum corneum and delivering drugs into deeper layers of the skin, thereby enhancing topical drug delivery. The present study aimed to formulate and evaluate a coumarin loaded transfersomal gel for improved topical delivery. Coumarin loaded transferosomes were prepared using the thin-film hydration method with phospholipids and an edge activator. The prepared vesicles were evaluated for vesicle size, polydispersity index, zeta potential and percentage drug content. The optimized transfersomal formulation was incorporated into a Carbopol gel base and further evaluated for appearance, pH, viscosity, spreadability, drug content, in vitro drug release and ex vivo skin permeation. The optimized transfersomal gel exhibited uniform appearance, suitable pH, good spreadability and sustained drug release with enhanced skin permeation compared to the plain coumarin gel. The findings suggest that the coumarin-loaded transfersomal gel is a promising topical drug delivery system capable of improving the therapeutic efficacy of coumarin by enhancing its skin penetration, prolonging drug release and increasing local drug availability. Therefore, transfersomal gel represents a potential carrier for the effective topical treatment of skin disorders.
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References
I. Kumar PK, Kumar RS. Review on transferosomes and transferosomal gels. Journal of Pharmaceutical Research International. 2021;33(43B):114-126. doi:10.9734/jpri/2021/v33i43B32532.
II. Chaudhari G, Morris S, Jain A. Nanoscale navigation: A review on transfersomes for transdermal drug delivery. International Journal of Research in Pharmacy and Allied Science. 2024;3(3):64-79.
III. Pahwa R, Pal S, Saroha K, Waliyan P, Kumar M. Transferosomes: Unique vesicular carriers for effective transdermal delivery. Journal of Applied Pharmaceutical Science. 2021;11(5):1-8. doi:10.7324/JAPS.2021.110501.
IV. Chauhan N, Singh P, Nayak S. Elastic vesicles in dermatological and transdermal drug delivery: A comprehensive review on transfersomes. Panacea Journal of Pharmacy and Pharmaceutical Sciences. 2025;14(2).
V. El Zaafarany GM, Awad GAS, Holayel SA, Mortada ND. Role of edge activators and surface charge in developing ultradeformable vesicles with enhanced skin delivery. International Journal of Pharmaceutics. 2010;397(1-2):164-172.
VI. Patel R, Patel H, Baria A. Formulation and evaluation of transferosomal gel for topical delivery of therapeutic agents. International Journal of Pharmaceutical Research and Development. 2012;4(2):50-56.
VII. Jain S, Tiwary AK, Sapra B, Jain NK. Formulation and evaluation of ethosomes for transdermal delivery of lamivudine. AAPS PharmSciTech. 2007;8(4).
VIII. Boinpally RR, Zhou SL, Poondru S, Devraj G, Jasti BR. Transdermal delivery of hydrocortisone through hairless mouse skin using microemulsions. Journal of Pharmaceutical Sciences. 2003;92(4):892-900.
IX. Bhattacharyya S, Lakshmanan KT, Muthukumar A. Formulation and evaluation of a transferosomal gel of famciclovir for transdermal use. Turkish Journal of Pharmaceutical Sciences. 2024;21(4):303-312. doi:10.4274/tjps.galenos.2023.46735.
X. Annunziata F, Pinna C, Dallavalle S, Tamborini L, Pinto A. An overview of coumarin as a versatile and readily accessible scaffold with broad-ranging biological activities. International Journal of Molecular Sciences. 2020;21(13):4618. doi:10.3390/ijms21134618.
XI. Opatha SAT, Titapiwatanakun V, Chutoprapat R. Transfersomes: A promising nanoencapsulation technique for transdermal drug delivery. Pharmaceutics. 2020;12(9):855. doi:10.3390/pharmaceutics12090855.
XII. Marcolan M, Martins PA, Pedrosa VA, Magini MRR, de Oliveira HPM, Codognoto L. Spectrofluorimetric determination of coumarin in commercial tablets. Journal of Fluorescence. 2011;21(2):733-738. doi:10.1007/s10895-010-0763-5.
XIII. Arivazhagan M, Sambathkumar K, Jeyavijayan S. Density functional theory study of FTIR and FT-Raman spectra of 7-acetoxy-4-methyl coumarin. Indian Journal of Pure & Applied Physics. 2010;48(10):716-722.
XIV. Kuş N, Breda S, Reva I, Tasal E, Öğretir C, Fausto R. FTIR spectroscopic and theoretical study of the photochemistry of matrix-isolated coumarin. Photochemistry and Photobiology. 2007;83:1237-1253.
XV. Jangde R, Gupta A, Pooja D. Formulation and evaluation of transferosomal gel for enhanced topical delivery: A review. International Journal of Pharmaceutical Sciences and Research. 2020;11(5):2145-2153.
XVI. Cevc G, Blume G. Lipid vesicles penetrate into intact skin owing to the transdermal osmotic gradients and hydration force. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1992;1104(1):226-232.
XVII. Basha BN, Prakasam K, Goli D. Formulation and evaluation of gel containing transferosomal vesicles for topical drug delivery. International Journal of Pharmacy and Pharmaceutical Sciences. 2011;3(4):109-112.
XVIII. Benson HAE. Transfersomes for transdermal drug delivery. Expert Opinion on Drug Delivery. 2006;3(6):727-737.
XIX. Opatha SAT, Titapiwatanakun V. Transfersomes: A promising nano delivery system for enhanced transdermal drug delivery. Asian Journal of Pharmaceutical Sciences. 2020;15(4):437-452.
XX. Mishra D, Garg M, Dubey V, Jain S, Jain NK. Elastic liposomes mediated transdermal delivery of an anti-hypertensive agent: Propranolol hydrochloride. Journal of Pharmacy and Pharmacology. 2007;59(10):1433-1438.
XXI. Jain S, Tiwary AK, Jain NK. PEGylated elastic liposomal formulation for topical delivery of tacrolimus: Preparation and evaluation. Drug Development and Industrial Pharmacy. 2007;33(3):255-266.